Vi(MA)2C

Vision-based Microscope Automation for Microbial Arena Composition

Microscopy image of green and brown rod-shaped microbial cells arranged in distinct regions, illustrating the controlled spatial organization of microbial communities in the Helmholtz Imaging project Vi(MA)²C.
Image: Vi(MA)2C | info

Vi(MA)²C pioneers vision-based automation for the controlled composition and dynamic reconfiguration of microbial arenas in microfluidic live-cell imaging.

The project combines high-speed computer vision for real-time segmentation and tracking with optical tweezers to create closed-loop systems that sense, analyze, and manipulate living microbial communities on the fly.

Living microbes and functional microgels acting as sensors, nutrient reservoirs, or carriers are autonomously positioned through precise pick-and-place operations, enabling intentional spatiotemporal scene design at single-cell resolution.

Vi(MA)²C advances towards active, high-speed (up to 20 fps) manipulation and autonomous experimentation. In doing so, it transforms researchers from passive observers into active designers of microbial environments.

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