In collaboration with the Center for Structural Systems biology, Helmholtz Imaging has developed and trained a convolutional neural network for the picking of instances of proteins in cryoelectrontomograms (CryoET). The so picked instances are subsequently used to reconstruct a 3D structure of the proteins using subtomogram averaging methods. The novel picking methods exploit the well known YOLO object detection algorithm and thus can analyse an entire tomogram in about 1 second.